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TitleStructures of transcription preinitiation complex engaged with the +1 nucleosome.
Journal, issue, pagesNat Struct Mol Biol, Vol. 30, Issue 2, Page 226-232, Year 2023
Publish dateNov 21, 2022
AuthorsHaibo Wang / Sandra Schilbach / Momchil Ninov / Henning Urlaub / Patrick Cramer /
PubMed AbstractThe preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on ...The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on different promoters, but did not address how the PIC assembles within chromatin. In the yeast Saccharomyces cerevisiae, PIC assembly occurs adjacent to the +1 nucleosome that is located downstream of the core promoter. Here we present cryo-EM structures of the yeast PIC bound to promoter DNA and the +1 nucleosome located at three different positions. The general transcription factor TFIIH engages with the incoming downstream nucleosome and its translocase subunit Ssl2 (XPB in human TFIIH) drives the rotation of the +1 nucleosome leading to partial detachment of nucleosomal DNA and intimate interactions between TFIIH and the nucleosome. The structures provide insights into how transcription initiation can be influenced by the +1 nucleosome and may explain why the transcription start site is often located roughly 60 base pairs upstream of the dyad of the +1 nucleosome in yeast.
External linksNat Struct Mol Biol / PubMed:36411341 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 6.6 Å
Structure data

14927

7zs9

EMDB-14927, PDB-7zs9:
Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome (complex A)
Method: EM (single particle) / Resolution: 3.1 Å

14928

7zsa

EMDB-14928, PDB-7zsa:
Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome and NTP (complex B)
Method: EM (single particle) / Resolution: 4.0 Å

14929

7zsb

EMDB-14929, PDB-7zsb:
Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome and NTP, complex C
Method: EM (single particle) / Resolution: 6.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

Source
  • Saccharomyces killer virus M2-4
  • saccharomyces cerevisiae (brewer's yeast)
  • xenopus laevis (African clawed frog)
  • synthetic construct (others)
  • baker's yeast (brewer's yeast)
KeywordsTRANSCRIPTION / PIC / nucleosome / TF

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