[English] 日本語
Yorodumi Papers
- Database of articles cited by EMDB/PDB/SASBDB data -

+
Search query

Keywords
Structure methods
Author
Journal
IF

-
Structure paper

TitleMultishot tomography for high-resolution in situ subtomogram averaging.
Journal, issue, pagesJ Struct Biol, Vol. 215, Issue 1, Page 107911, Year 2023
Publish dateNov 4, 2022
AuthorsS Khavnekar / W Wan / P Majumder / W Wietrzynski / P S Erdmann / J M Plitzko /
PubMed AbstractCryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can ...Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET can be slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic acquisition scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, which yields sub-nanometer in situ STA averages.
External linksJ Struct Biol / PubMed:36343843
MethodsEM (subtomogram averaging)
Resolution4.7 - 8.8 Å
Structure data

EMDB-16139: E.coli 70S ribosome subtomogram average from multishot acquisition on cryo-FIB lamellae
Method: EM (subtomogram averaging) / Resolution: 8.8 Å

EMDB-16162: E.coli 70S ribosome subtomogram average from singleshot acquisition on mixed Ribosome-Proteasome sample
Method: EM (subtomogram averaging) / Resolution: 7.8 Å

EMDB-16165: E.coli 70S ribosome subtomogram average from multishot acquisition on mixed Ribosome-Proteasome sample
Method: EM (subtomogram averaging) / Resolution: 8.3 Å

EMDB-16180: 20S Proteasome subtomogram average from Singleshot acquisition on mixed Riobsome-Proteasome sample
Method: EM (subtomogram averaging) / Resolution: 4.7 Å

EMDB-16181: 20S Proteasome subtomogram average from multishot tomography acquisition on mixed Ribosome-Proteasome sample
Method: EM (subtomogram averaging) / Resolution: 4.7 Å

Source
  • Escherichia coli (E. coli)
  • Thermoplasma acidophilum (acidophilic)

+
About Yorodumi Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi Papers

Database of articles cited by EMDB/PDB/SASBDB data

  • Database of articles cited by EMDB, PDB, and SASBDB entries
  • Using PubMed data

Related info.:EMDB / PDB / SASBDB / Yorodumi / EMN Papers / Changes in new EM Navigator and Yorodumi

Read more