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Structure paper

TitleInsights into the ribosome function from the structures of non-arrested ribosome-nascent chain complexes.
Journal, issue, pagesNat. Chem., Vol. 15, Page 143-153, Year 2023
Publish dateMay 18, 2022 (structure data deposition date)
AuthorsSyroegin, E.A. / Aleksandrova, E.V. / Polikanov, Y.S.
External linksNat. Chem. / PubMed:36316410
MethodsX-ray diffraction
Resolution2.3 - 2.5 Å
Structure data

PDB-8cvj:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMSEAC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-8cvk:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-8cvl:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMTHSMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.30A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
  • thermus thermophilus hb8 (bacteria)
  • escherichia virus t4
KeywordsRIBOSOME/RNA / 70S ribosome / non-hydrolyzable / aminoacyl-tRNA / peptidyl-tRNA / formylation / pre-attack state / transpeptidation / peptidyl transferase center / nascent peptide exit tunnel / RIBOSOME / RIBOSOME-RNA complex / peptidyl-tRNA; formylation

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