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TitleEvolution of increased complexity and specificity at the dawn of form I Rubiscos.
Journal, issue, pagesScience, Vol. 378, Issue 6616, Page 155-160, Year 2022
Publish dateOct 14, 2022
AuthorsLuca Schulz / Zhijun Guo / Jan Zarzycki / Wieland Steinchen / Jan M Schuller / Thomas Heimerl / Simone Prinz / Oliver Mueller-Cajar / Tobias J Erb / Georg K A Hochberg /
PubMed AbstractThe evolution of ribulose-1,5-bisphosphate carboxylase/oxygenases (Rubiscos) that discriminate strongly between their substrate carbon dioxide and the undesired side substrate dioxygen was an ...The evolution of ribulose-1,5-bisphosphate carboxylase/oxygenases (Rubiscos) that discriminate strongly between their substrate carbon dioxide and the undesired side substrate dioxygen was an important event for photosynthetic organisms adapting to an oxygenated environment. We use ancestral sequence reconstruction to recapitulate this event. We show that Rubisco increased its specificity and carboxylation efficiency through the gain of an accessory subunit before atmospheric oxygen was present. Using structural and biochemical approaches, we retrace how this subunit was gained and became essential. Our work illuminates the emergence of an adaptation to rising ambient oxygen levels, provides a template for investigating the function of interactions that have remained elusive because of their essentiality, and sheds light on the determinants of specificity in Rubisco.
External linksScience / PubMed:36227987
MethodsEM (single particle) / X-ray diffraction
Resolution1.8 - 3.0 Å
Structure data

14178

7qvi

EMDB-14178, PDB-7qvi:
Fiber-forming RubisCO derived from ancestral sequence reconstruction and rational engineering
Method: EM (single particle) / Resolution: 3.0 Å

PDB-7qsv:
L8-complex forming RubisCO derived from ancestral sequence reconstruction of the last common ancestor of Form I'' and Form I RubisCOs
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7qsw:
L8S8-complex forming RubisCO derived from ancestral sequence reconstruction of the last common ancestor of SSU-bearing Form I RubisCOs
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-7qsx:
Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8 complex
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-7qsy:
Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8S8 complex
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7qsz:
Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8 complex with substitution e170N
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-7qt1:
Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8S8 complex with substitution e170N
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-CAP:
2-CARBOXYARABINITOL-1,5-DIPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • synthetic construct (others)
KeywordsLYASE / Ribulose 1 / 5-bisphosphate carboxylase/oxydase / RubisCO

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