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Structure paper

TitleNMR-guided directed evolution.
Journal, issue, pagesNature, Vol. 610, Page 389-393, Year 2022
Publish dateNov 2, 2021 (structure data deposition date)
AuthorsBhattacharya, S. / Margheritis, E.G. / Takahashi, K. / Kulesha, A. / D'Souza, A. / Kim, I. / Yoon, J.H. / Tame, J.R.H. / Volkov, A.N. / Makhlynets, O.V. / Korendovych, I.V.
External linksNature / PubMed:36198791
MethodsX-ray diffraction
Resolution1.4 - 1.95 Å
Structure data

PDB-7vuc:
Myoglobin mutant L29I/H64G/V68A
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-7vur:
Crystal structure of AlleyCat9 with calcium but no inhibitor
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7vus:
Crystal structure of AlleyCat9 with 5-nitro-benzotriazole
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7vut:
Crystal structure of AlleyCat10
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7vuu:
Crystal structure of AlleyCat10 with inhibitor
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-IMD:
IMIDAZOLE

ChemComp-HOH:
WATER

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-CA:
Unknown entry

ChemComp-3NY:
5-nitro-1H-benzotriazole

Source
  • physeter macrocephalus (sperm whale)
  • homo sapiens (human)
KeywordsBIOSYNTHETIC PROTEIN / Artificial / enzyme / myoglobin / calmodulin / BIOSYNTHETIC PROTEIN/INHIBITOR / BIOSYNTHETIC PROTEIN-INHIBITOR COMPLEX

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