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TitleDevelopmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System.
Journal, issue, pagesInfect Immun, Vol. 90, Issue 10, Page e0041022, Year 2022
Publish dateOct 20, 2022
AuthorsDonghyun Park / Samuel Steiner / Meng Shao / Craig R Roy / Jun Liu /
PubMed AbstractCoxiella burnetii is an obligate intracellular bacterial pathogen that has evolved a unique biphasic developmental cycle. The infectious form of C. burnetii is the dormant small cell variant (SCV), ...Coxiella burnetii is an obligate intracellular bacterial pathogen that has evolved a unique biphasic developmental cycle. The infectious form of C. burnetii is the dormant small cell variant (SCV), which transitions to a metabolically active large cell variant (LCV) that replicates inside the lysosome-derived host vacuole. A Dot/Icm type IV secretion system (T4SS), which can deliver over 100 effector proteins to host cells, is essential for the biogenesis of the vacuole and intracellular replication. How the distinct C. burnetii life cycle impacts the assembly and function of the Dot/Icm T4SS has remained unknown. Here, we combine advanced cryo-focused ion beam (cryo-FIB) milling and cryo-electron tomography (cryo-ET) imaging to visualize all developmental transitions and the assembly of the Dot/Icm T4SS . Importantly, assembled Dot/Icm machines were not present in the infectious SCV. The appearance of the assembled Dot/Icm machine correlated with the transition of the SCV to the LCV intracellularly. Furthermore, temporal characterization of C. burnetii morphological changes revealed regions of the inner membrane that invaginate to form tightly packed stacks during the LCV-to-SCV transition at late stages of infection, which may enable the SCV-to-LCV transition that occurs upon infection of a new host cell. Overall, these data establish how C. burnetii developmental transitions control critical bacterial processes to promote intracellular replication and transmission.
External linksInfect Immun / PubMed:36190257 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution11.0 Å
Structure data

EMDB-27105: Coxiella T4SS OMC
Method: EM (subtomogram averaging) / Resolution: 11.0 Å

EMDB-27106: Coxiella Small Cell Variant (SCV)
Method: EM (tomography)

EMDB-27108: Coxiella large cell variant (LCV)
Method: EM (tomography)

EMDB-27110: Intracellular Coxiella 48hpi
Method: EM (tomography)

EMDB-27111: Intracellular Coxiella 6hpi
Method: EM (tomography)

Source
  • Coxiella burnetii (bacteria)

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