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-Structure paper
タイトル | Elastic properties and shape of the Piezo dome underlying its mechanosensory function. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 119, Issue 40, Page e2208034119, Year 2022 |
掲載日 | 2022年10月4日 |
著者 | Christoph A Haselwandter / Yusong R Guo / Ziao Fu / Roderick MacKinnon / |
PubMed 要旨 | We show in the companion paper that the free membrane shape of lipid bilayer vesicles containing the mechanosensitive ion channel Piezo can be predicted, with no free parameters, from membrane ...We show in the companion paper that the free membrane shape of lipid bilayer vesicles containing the mechanosensitive ion channel Piezo can be predicted, with no free parameters, from membrane elasticity theory together with measurements of the protein geometry and vesicle size [C. A. Haselwandter, Y. R. Guo, Z. Fu, R. MacKinnon, , 10.1073/pnas.2208027119 (2022)]. Here we use these results to determine the force that the Piezo dome exerts on the free membrane and hence, that the free membrane exerts on the Piezo dome, for a range of vesicle sizes. From vesicle shape measurements alone, we thus obtain a force-distortion relationship for the Piezo dome, from which we deduce the Piezo dome's intrinsic radius of curvature, [Formula: see text] nm, and bending stiffness, [Formula: see text], in freestanding lipid bilayer membranes mimicking cell membranes. Applying these estimates to a spherical cap model of Piezo embedded in a lipid bilayer, we suggest that Piezo's intrinsic curvature, surrounding membrane footprint, small stiffness, and large area are the key properties of Piezo that give rise to low-threshold, high-sensitivity mechanical gating. |
リンク | Proc Natl Acad Sci U S A / PubMed:36166476 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-27567: Tomogram of Piezo1 vesicles EMDB-27568: Tomogram of Piezo1 vesicles EMDB-27569: Tomogram of Piezo1 vesicles EMDB-27570: Tomogram of Piezo1 vesicles EMDB-27571: Tomogram of Piezo1 vesicles |
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