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Structure paper

TitleMolecular basis for thermal stability and affinity in a VHH: Contribution of the framework region and its influence in the conformation of the CDR3.
Journal, issue, pagesProtein Sci., Vol. 31, Page e4450-e4450, Year 2022
Publish dateApr 20, 2022 (structure data deposition date)
AuthorsKinoshita, S. / Nakakido, M. / Mori, C. / Kuroda, D. / Caaveiro, J.M.M. / Tsumoto, K.
External linksProtein Sci. / PubMed:36153698
MethodsX-ray diffraction
Resolution1.65 - 1.7 Å
Structure data

PDB-7xl0:
Crystal structure of Vobarilizumab at 1.70 Angstrom
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7xl1:
Crystal structure of chimeric 7D12-Vob nanobody at 1.65 Angstrom
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-MLI:
MALONATE ION

Source
  • lama glama (llama)
KeywordsIMMUNE SYSTEM / nanobody VHH protein engineering protein stability surface plasmon resonance differential scanning calorimetry

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