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TitleStructural insights into thermostable direct hemolysin of Vibrio parahaemolyticus using single-particle cryo-EM.
Journal, issue, pagesProteins, Vol. 91, Issue 2, Page 137-146, Year 2023
Publish dateSep 8, 2022
AuthorsSuman Mishra / Nidhi Kundu / Ishika Pramanick / Anil Kumar / Kausik Chattopadhyay / Somnath Dutta /
PubMed AbstractThermostable direct hemolysin (TDH) is a ~19 kDa, hemolytic pore-forming toxin from the gram-negative marine bacterium Vibrio parahaemolyticus, one of the causative agents of seafood-borne acute ...Thermostable direct hemolysin (TDH) is a ~19 kDa, hemolytic pore-forming toxin from the gram-negative marine bacterium Vibrio parahaemolyticus, one of the causative agents of seafood-borne acute gastroenteritis and septicemia. Previous studies have established that TDH exists as a tetrameric assembly in physiological state; however, there is limited knowledge regarding the molecular arrangement of its disordered N-terminal region (NTR)-the absence of which has been shown to compromise TDH's hemolytic and cytotoxic abilities. In our current study, we have employed single-particle cryo-electron microscopy to resolve the solution-state structures of wild-type TDH and a TDH construct with deletion of the NTR (NTD), in order to investigate structural aspects of NTR on the overall tetrameric architecture. We observed that both TDH and NTD electron density maps, resolved at global resolutions of 4.5 and 4.2 Å, respectively, showed good correlation in their respective oligomeric architecture. Additionally, we were able to locate extra densities near the pore opening of TDH which might correspond to the disordered NTR. Surprisingly, under cryogenic conditions, we were also able to observe novel supramolecular assemblies of TDH tetramers, which we were able to resolve to 4.3 Å. We further investigated the tetrameric and inter-tetrameric interaction interfaces to elaborate upon the key residues involved in both TDH tetramers and TDH super assemblies. Our current structural study will aid in understanding the mechanistic aspects of this pore-forming toxin and the role of its disordered NTR in membrane interaction.
External linksProteins / PubMed:36000388
MethodsEM (single particle)
Resolution4.2 - 4.5 Å
Structure data

EMDB-33411: Supramolecular assembly of Thermostable Direct Hemolysin from Vibrio parahaemolyticus
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-33412: Wild type Thermostable Direct Hemolysin (TDH) from Vibrio parahaemolyticus
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-33413: N-terminal deleted (NTD) Thermostable Direct Hemolysin from Vibrio parahaemolyticus
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • Vibrio parahaemolyticus (bacteria)

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