EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	SARS-CoV-2 variants of concern: spike protein mutational analysis and epitope for broad neutralization.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 4696, Year 2022
掲載日	2022年8月18日
著者	Dhiraj Mannar / James W Saville / Zehua Sun / Xing Zhu / Michelle M Marti / Shanti S Srivastava / Alison M Berezuk / Steven Zhou / Katharine S Tuttle / Michele D Sobolewski / Andrew Kim / Benjamin R Treat / Priscila Mayrelle Da Silva Castanha / Jana L Jacobs / Simon M Barratt-Boyes / John W Mellors / Dimiter S Dimitrov / Wei Li / Sriram Subramaniam /
PubMed 要旨	Mutations in the spike glycoproteins of SARS-CoV-2 variants of concern have independently been shown to enhance aspects of spike protein fitness. Here, we describe an antibody fragment (V ab6) that ...Mutations in the spike glycoproteins of SARS-CoV-2 variants of concern have independently been shown to enhance aspects of spike protein fitness. Here, we describe an antibody fragment (V ab6) that neutralizes all major variants including the recently emerged BA.1 and BA.2 Omicron subvariants, with a unique mode of binding revealed by cryo-EM studies. Further, we provide a comparative analysis of the mutational effects within previously emerged variant spikes and identify the structural role of mutations within the NTD and RBD in evading antibody neutralization. Our analysis shows that the highly mutated Gamma N-terminal domain exhibits considerable structural rearrangements, partially explaining its decreased neutralization by convalescent sera. Our results provide mechanistic insights into the structural, functional, and antigenic consequences of SARS-CoV-2 spike mutations and highlight a spike protein vulnerability that may be exploited to achieve broad protection against circulating variants.
リンク	Nat Commun / PubMed:35982054 / PubMed Central
手法	EM (単粒子)
解像度	2.16 - 3.21 Å
構造データ	27502 8dli EMDB-27502, PDB-8dli: Cryo-EM structure of SARS-CoV-2 Alpha (B.1.1.7) spike protein 手法: EM (単粒子) / 解像度: 2.56 Å 27503 8dlj EMDB-27503, PDB-8dlj: Cryo-EM structure of SARS-CoV-2 Alpha (B.1.1.7) spike protein in complex with human ACE2 手法: EM (単粒子) / 解像度: 2.91 Å 27504 8dlk EMDB-27504, PDB-8dlk: Cryo-EM structure of SARS-CoV-2 Alpha (B.1.1.7) spike protein in complex with human ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.04 Å 27505 8dll EMDB-27505, PDB-8dll: Cryo-EM structure of SARS-CoV-2 Beta (B.1.351) spike protein 手法: EM (単粒子) / 解像度: 2.56 Å 27506 8dlm EMDB-27506, PDB-8dlm: Cryo-EM structure of SARS-CoV-2 Beta (B.1.351) spike protein in complex with human ACE2 手法: EM (単粒子) / 解像度: 2.89 Å 27507 8dln EMDB-27507, PDB-8dln: Cryo-EM structure of SARS-CoV-2 Beta (B.1.351) spike protein in complex with human ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.04 Å 27508 8dlo EMDB-27508, PDB-8dlo: Cryo-EM structure of SARS-CoV-2 Gamma (P.1) spike protein 手法: EM (単粒子) / 解像度: 2.25 Å 27509 8dlp EMDB-27509, PDB-8dlp: Cryo-EM structure of SARS-CoV-2 Gamma (P.1) spike protein in complex with human ACE2 手法: EM (単粒子) / 解像度: 2.64 Å 27510 8dlq EMDB-27510, PDB-8dlq: Cryo-EM structure of SARS-CoV-2 Gamma (P.1) spike protein in complex with human ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 2.77 Å EMDB-27511: Cryo-EM structure of SARS-CoV-2 Gamma (P.1) spike protein in complex with Fab 4-8 手法: EM (単粒子) / 解像度: 2.59 Å 27512 8dlr EMDB-27512, PDB-8dlr: Cryo-EM structure of SARS-CoV-2 Gamma (P.1) spike protein in complex with Fab 4-8 (focused refinement of NTD and 4-8) 手法: EM (単粒子) / 解像度: 2.51 Å EMDB-27513: Cryo-EM structure of SARS-CoV-2 Gamma (P.1) spike protein in complex with Fab 4A8 手法: EM (単粒子) / 解像度: 2.59 Å 27514 8dls EMDB-27514, PDB-8dls: Cryo-EM structure of SARS-CoV-2 Gamma (P.1) spike protein in complex with Fab 4A8 (focused refinement of NTD and 4A8) 手法: EM (単粒子) / 解像度: 2.66 Å 27515 8dlt EMDB-27515, PDB-8dlt: Cryo-EM structure of SARS-CoV-2 Epsilon (B.1.429) spike protein 手法: EM (単粒子) / 解像度: 2.4 Å 27516 8dlu EMDB-27516, PDB-8dlu: Cryo-EM structure of SARS-CoV-2 Epsilon (B.1.429) spike protein in complex with human ACE2 手法: EM (単粒子) / 解像度: 3.14 Å 27517 8dlv EMDB-27517, PDB-8dlv: Cryo-EM structure of SARS-CoV-2 Epsilon (B.1.429) spike protein in complex with human ACE2 (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.11 Å 27518 8dlw EMDB-27518, PDB-8dlw: Cryo-EM structure of SARS-CoV-2 Epsilon (B.1.429) spike protein in complex with Fab S2M11 手法: EM (単粒子) / 解像度: 2.16 Å 27519 8dlx EMDB-27519, PDB-8dlx: Cryo-EM structure of SARS-CoV-2 Epsilon (B.1.429) spike protein in complex with VH ab6 手法: EM (単粒子) / 解像度: 2.45 Å 27520 8dly EMDB-27520, PDB-8dly: Cryo-EM structure of SARS-CoV-2 Epsilon (B.1.429) spike protein in complex with VH ab6 (focused refinement of NTD and VH ab6) 手法: EM (単粒子) / 解像度: 3.0 Å 27521 8dlz EMDB-27521, PDB-8dlz: Cryo-EM structure of SARS-CoV-2 D614G spike protein in complex with VH ab6 手法: EM (単粒子) / 解像度: 2.57 Å 27522 8dm0 EMDB-27522, PDB-8dm0: Cryo-EM structure of SARS-CoV-2 D614G spike protein in complex with VH ab6 (focused refinement of NTD and VH ab6) 手法: EM (単粒子) / 解像度: 3.21 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN / SARS-CoV-2 / glycoprotein / fusion protein / Viral Protein-Immune System complex / Alpha / B.1.1.7 / Viral Protein/Hydrolase / ACE2 / Viral Protein-Hydrolase complex / Beta / B.1.351 / Gamma / P.1 / 4-8 / 4A8 / Epsilon / B.1.429 / S2M11 / VH ab6 / VIRAL PROTEIN/IMMUNE SYSTEM