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TitleVisualizing molecular interactions that determine assembly of a bullet-shaped vesicular stomatitis virus particle.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 4802, Year 2022
Publish dateAug 15, 2022
AuthorsSimon Jenni / Joshua A Horwitz / Louis-Marie Bloyet / Sean P J Whelan / Stephen C Harrison /
PubMed AbstractVesicular stomatitis virus (VSV) is a negative-strand RNA virus with a non-segmented genome, closely related to rabies virus. Both have characteristic bullet-like shapes. We report the structure of ...Vesicular stomatitis virus (VSV) is a negative-strand RNA virus with a non-segmented genome, closely related to rabies virus. Both have characteristic bullet-like shapes. We report the structure of intact, infectious VSV particles determined by cryogenic electron microscopy. By compensating for polymorphism among viral particles with computational classification, we obtained a reconstruction of the shaft ("trunk") at 3.5 Å resolution, with lower resolution for the rounded tip. The ribonucleoprotein (RNP), genomic RNA complexed with nucleoprotein (N), curls into a dome-like structure with about eight gradually expanding turns before transitioning into the regular helical trunk. Two layers of matrix (M) protein link the RNP with the membrane. Radial inter-layer subunit contacts are fixed within single RNA-N-M1-M2 modules, but flexible lateral and axial interactions allow assembly of polymorphic virions. Together with published structures of recombinant N in various states, our results suggest a mechanism for membrane-coupled self-assembly of VSV and its relatives.
External linksNat Commun / PubMed:35970826 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution3.5 - 4.1 Å
Structure data

26602

7umk

EMDB-26602, PDB-7umk:
Structure of vesicular stomatitis virus (helical reconstruction, 4.1 A resolution)
Method: EM (helical sym.) / Resolution: 4.1 Å

26603

7uml

EMDB-26603, PDB-7uml:
Structure of vesicular stomatitis virus (local reconstruction, 3.5 A resolution)
Method: EM (single particle) / Resolution: 3.5 Å

Source
  • vesicular stomatitis indiana virus
KeywordsVIRUS / Vesicular stomatitis virus / bullet-shaped particle / Rhrabdovirus / nucleocapsid / helical reconstruction / cryo electron microscopy / nucleoprotein / matrix protein / RNA / assembly / VIRAL PROTEIN/RNA / VIRAL PROTEIN-RNA complex

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