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TitleAncestral Sequence Reconstruction Identifies Structural Changes Underlying the Evolution of Ideonella sakaiensis PETase and Variants with Improved Stability and Activity.
Journal, issue, pagesBiochemistry, Vol. 62, Page 437-450, Year 2023
Publish dateMay 11, 2022 (structure data deposition date)
AuthorsJoho, Y. / Vongsouthi, V. / Spence, M.A. / Ton, J. / Gomez, C. / Tan, L.L. / Kaczmarski, J.A. / Caputo, A.T. / Royan, S. / Jackson, C.J. / Ardevol, A.
External linksBiochemistry / PubMed:35951410
MethodsX-ray diffraction
Resolution1.3 Å
Structure data

PDB-8cru:
PETase Ancestral Sequence Reconstruction 008
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

Chemicals

ChemComp-CIT:
CITRIC ACID

ChemComp-HOH:
WATER

Source
  • ideonella sakaiensis (bacteria)
KeywordsHYDROLASE / PETase / ancestral sequence reconstruction

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