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TitleProgrammable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity.
Journal, issue, pagesNat Commun, Vol. 13, Page 4624-4624, Year 2022
Publish dateJun 26, 2021 (structure data deposition date)
AuthorsLisitskaya, L. / Shin, Y. / Agapov, A. / Olina, A. / Kropocheva, E. / Ryazansky, S. / Aravin, A.A. / Esyunina, D. / Murakami, K.S. / Kulbachinskiy, A.
External linksNat Commun / PubMed:35941106
MethodsX-ray diffraction
Resolution2.5 - 3.28 Å
Structure data

PDB-7r8f:
Crystal structure of Pseudooceanicola lipolyticus Argonaute
Method: X-RAY DIFFRACTION / Resolution: 3.16 Å

PDB-7r8g:
Crystal structure of Pseudooceanicola lipolyticus Argonaute bound to 5' OH guide DNA
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7r8h:
Crystal structure of Pseudooceanicola lipolyticus Argonaute bound to 5' p guide DNA
Method: X-RAY DIFFRACTION / Resolution: 2.54 Å

PDB-7r8j:
Crystal structure of Pseudooceanicola lipolyticus Argonaute bound to 5' p guide DNA in the presence of Mg2+
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-7r8k:
Crystal structure of Pseudooceanicola lipolyticus Argonaute (SeMet labeled protein)
Method: X-RAY DIFFRACTION / Resolution: 3.28 Å

Source
  • pseudooceanicola lipolyticus (bacteria)
KeywordsHYDROLASE / argonaute / Ago / DNA guide / RNA cleavage / HYDROLASE/DNA / HYDROLASE-DNA complex

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