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-Structure paper
Title | In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains. |
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Journal, issue, pages | Science, Vol. 377, Issue 6605, Page 543-548, Year 2022 |
Publish date | Jul 29, 2022 |
Authors | Hugo van den Hoek / Nikolai Klena / Mareike A Jordan / Gonzalo Alvarez Viar / Ricardo D Righetto / Miroslava Schaffer / Philipp S Erdmann / William Wan / Stefan Geimer / Jürgen M Plitzko / Wolfgang Baumeister / Gaia Pigino / Virginie Hamel / Paul Guichard / Benjamin D Engel / |
PubMed Abstract | The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the ...The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the entry of large intraflagellar transport (IFT) trains, which carry cargo proteins required for ciliary assembly and maintenance. However, the native architecture of the ciliary base and the process of IFT train assembly remain unresolved. In this work, we used in situ cryo-electron tomography to reveal native structures of the transition zone region and assembling IFT trains at the ciliary base in . We combined this direct cellular visualization with ultrastructure expansion microscopy to describe the front-to-back stepwise assembly of IFT trains: IFT-B forms the backbone, onto which bind IFT-A, dynein-1b, and finally kinesin-2 before entry into the cilium. |
External links | Science / PubMed:35901159 |
Methods | EM (subtomogram averaging) / EM (tomography) |
Resolution | 24.4 - 34.7 Å |
Structure data | EMDB-15252: In situ subtomogram average of the C. reinhardtii stellate at the ciliary transition zone EMDB-15253: In situ subtomogram average of the C. reinhardtii Y-link at the ciliary transition zone EMDB-15254: In situ subtomogram average of the C. reinhardtii MTD sleeve at the ciliary transition zone EMDB-15255: In situ subtomogram average of the C. reinhardtii MTD at the ciliary transition zone EMDB-15256: Composite map of the C. reinhardtii ciliary transition zone (structures attached to a single MTD) from in situ subtomogram averaging EMDB-15257: Composite map of the C. reinhardtii ciliary transition zone (full 9-fold assembly) from in situ subtomogram averaging EMDB-15258: In situ subtomogram average of C. reinhardtii IFT-B at the ciliary transition zone EMDB-15259: In situ subtomogram average of C. reinhardtii IFT-A at the ciliary transition zone EMDB-15260: In situ subtomogram average of C. reinhardtii dynein-1b at the ciliary transition zone EMDB-15261: Composite map of the C. reinhardtii IFT (segment of a fully assembled train) at the ciliary transition zone EMDB-15262: Tomogram #3 of the C. reinhardtii ciliary transition zone EMDB-15263: Tomogram #12 of the C. reinhardtii ciliary transition zone |
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