EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Modulating co-translational protein folding by rational design and ribosome engineering.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 4243, Year 2022
掲載日	2022年7月22日
著者	Minkoo Ahn / Tomasz Włodarski / Alkistis Mitropoulou / Sammy H S Chan / Haneesh Sidhu / Elena Plessa / Thomas A Becker / Nediljko Budisa / Christopher A Waudby / Roland Beckmann / Anaïs M E Cassaignau / Lisa D Cabrita / John Christodoulou /
PubMed 要旨	Co-translational folding is a fundamental process for the efficient biosynthesis of nascent polypeptides that emerge through the ribosome exit tunnel. To understand how this process is modulated by ...Co-translational folding is a fundamental process for the efficient biosynthesis of nascent polypeptides that emerge through the ribosome exit tunnel. To understand how this process is modulated by the shape and surface of the narrow tunnel, we have rationally engineered three exit tunnel protein loops (uL22, uL23 and uL24) of the 70S ribosome by CRISPR/Cas9 gene editing, and studied the co-translational folding of an immunoglobulin-like filamin domain (FLN5). Our thermodynamics measurements employing F/N/methyl-TROSY NMR spectroscopy together with cryo-EM and molecular dynamics simulations reveal how the variations in the lengths of the loops present across species exert their distinct effects on the free energy of FLN5 folding. A concerted interplay of the uL23 and uL24 loops is sufficient to alter co-translational folding energetics, which we highlight by the opposite folding outcomes resulting from their extensions. These subtle modulations occur through a combination of the steric effects relating to the shape of the tunnel, the dynamic interactions between the ribosome surface and the unfolded nascent chain, and its altered exit pathway within the vestibule. These results illustrate the role of the exit tunnel structure in co-translational folding, and provide principles for how to remodel it to elicit a desired folding outcome.
リンク	Nat Commun / PubMed:35869078 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 2.75 Å
構造データ	14454 7z20 EMDB-14454, PDB-7z20: 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia and a stalled filamin domain 5 nascent chain 手法: EM (単粒子) / 解像度: 2.29 Å 14846 7zod EMDB-14846, PDB-7zod: 70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia 手法: EM (単粒子) / 解像度: 2.56 Å 14850 7zp8 EMDB-14850, PDB-7zp8: 70S E. coli ribosome with a stalled filamin domain 5 nascent chain 手法: EM (単粒子) / 解像度: 2.2 Å 14864 7zq5 EMDB-14864, PDB-7zq5: 70S E. coli ribosome with truncated uL23 and uL24 loops 手法: EM (単粒子) / 解像度: 2.7 Å 14865 7zq6 EMDB-14865, PDB-7zq6: 70S E. coli ribosome with truncated uL23 and uL24 loops and a stalled filamin domain 5 nascent chain 手法: EM (単粒子) / 解像度: 2.75 Å
由来	Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌) escherichia coli (大腸菌) dictyostelium discoideum (キイロタマホコリカビ)
キーワード	RIBOSOME / exit tunnel / structural modification / ribosomal protein / folded nascent chain