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TitleStructure-guided engineering of key amino acids in UGT85B1 controlling substrate and stereo-specificity in aromatic cyanogenic glucoside biosynthesis.
Journal, issue, pagesPlant J., Vol. 111, Page 1539-1549, Year 2022
Publish dateMar 31, 2022 (structure data deposition date)
AuthorsDel Giudice, R. / Putkaradze, N. / Dos Santos, B.M. / Hansen, C.C. / Crocoll, C. / Motawia, M.S. / Fredslund, F. / Laursen, T. / Welner, D.H.
External linksPlant J. / PubMed:35819080
MethodsX-ray diffraction
Resolution1.42 - 1.5 Å
Structure data

PDB-7zer:
Crystal structure of UGT85B1 from Sorghum bicolor in complex with UDP
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

PDB-7zf0:
Crystal structure of UGT85B1 from Sorghum bicolor in complex with UDP and p-hydroxymandelonitrile
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-DHR:
(2S)-HYDROXY(4-HYDROXYPHENYL)ETHANENITRILE

Source
  • sorghum bicolor (sorghum)
KeywordsTRANSFERASE / glycosyltransferase / cyanogenic / dhurrin biosynthesis / GT-B fold

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