EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM reveals mechanisms of angiotensin I-converting enzyme allostery and dimerization.
ジャーナル・号・ページ	EMBO J, Vol. 41, Issue 16, Page e110550, Year 2022
掲載日	2022年7月12日
著者	Lizelle Lubbe / Bryan Trevor Sewell / Jeremy D Woodward / Edward D Sturrock /
PubMed 要旨	Hypertension (high blood pressure) is a major risk factor for cardiovascular disease, which is the leading cause of death worldwide. The somatic isoform of angiotensin I-converting enzyme (sACE) ...Hypertension (high blood pressure) is a major risk factor for cardiovascular disease, which is the leading cause of death worldwide. The somatic isoform of angiotensin I-converting enzyme (sACE) plays a critical role in blood pressure regulation, and ACE inhibitors are thus widely used to treat hypertension and cardiovascular disease. Our current understanding of sACE structure, dynamics, function, and inhibition has been limited because truncated, minimally glycosylated forms of sACE are typically used for X-ray crystallography and molecular dynamics simulations. Here, we report the first cryo-EM structures of full-length, glycosylated, soluble sACE (sACE ). Both monomeric and dimeric forms of the highly flexible apo enzyme were reconstructed from a single dataset. The N- and C-terminal domains of monomeric sACE were resolved at 3.7 and 4.1 Å, respectively, while the interacting N-terminal domains responsible for dimer formation were resolved at 3.8 Å. Mechanisms are proposed for intradomain hinging, cooperativity, and homodimerization. Furthermore, the observation that both domains were in the open conformation has implications for the design of sACE modulators.
リンク	EMBO J / PubMed:35818993 / PubMed Central
手法	EM (単粒子)
解像度	3.63 - 4.34 Å
構造データ	13797 7q3y EMDB-13797, PDB-7q3y: Structure of full-length, monomeric, soluble somatic angiotensin I-converting enzyme showing the N- and C-terminal ellipsoid domains 手法: EM (単粒子) / 解像度: 4.34 Å 13799 7q49 EMDB-13799, PDB-7q49: Local refinement structure of the N-domain of full-length, monomeric, soluble somatic angiotensin I-converting enzyme 手法: EM (単粒子) / 解像度: 3.72 Å 13801 7q4c EMDB-13801, PDB-7q4c: Local refinement structure of the C-domain of full-length, monomeric, soluble somatic angiotensin I-converting enzyme 手法: EM (単粒子) / 解像度: 4.08 Å EMDB-13802: Structure of full-length, dimeric, soluble somatic angiotensin I-converting enzyme 手法: EM (単粒子) / 解像度: 3.79 Å 13803 7q4d EMDB-13803, PDB-7q4d: Local refinement structure of the two interacting N-domains of full-length, dimeric, soluble somatic angiotensin I-converting enzyme 手法: EM (単粒子) / 解像度: 3.78 Å 13804 7q4e EMDB-13804, PDB-7q4e: Local refinement structure of a single N-domain of full-length, dimeric, soluble somatic angiotensin I-converting enzyme 手法: EM (単粒子) / 解像度: 3.63 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-CL: Unknown entry / クロリド ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-HOH: WATER
由来	homo sapiens (ヒト)
キーワード	HYDROLASE / Zinc metalloprotease Dicarboxypeptidase Glycoprotein