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Structure paper

TitleUsing fungible biosensors to evolve improved alkaloid biosyntheses.
Journal, issue, pagesNat. Chem. Biol., Vol. 18, Page 981-989, Year 2022
Publish dateJun 4, 2021 (structure data deposition date)
Authorsd'Oelsnitz, S. / Kim, W. / Burkholder, N.T. / Javanmardi, K. / Thyer, R. / Zhang, Y. / Alper, H.S. / Ellington, A.D.
External linksNat. Chem. Biol. / PubMed:35799063
MethodsX-ray diffraction
Resolution1.6 - 2.21 Å
Structure data

PDB-7n4w:
Complex structure of ROTU4 with rotundine
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

PDB-7n4z:
Complex structure of NOS4 with noscapine
Method: X-RAY DIFFRACTION / Resolution: 2.21 Å

PDB-7n53:
Complex structure of PAP4 with papaverine
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-7n54:
Complex structure of GLAU4 with glaucine
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-P4V:
rotundine / alkaloid*YM

ChemComp-HOH:
WATER

ChemComp-08N:
noscapine / alkaloid*YM

ChemComp-EV1:
1-(3,4-DIMETHOXYBENZYL)-6,7-DIMETHOXYISOQUINOLINE / alkaloid*YM

ChemComp-08V:
glaucine / antiinflammatory, channel blocker, inhibitor, alkaloid*YM

Source
  • salmonella enterica subsp. enterica serovar typhimurium str. 14028s (bacteria)
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
KeywordsTRANSCRIPTION / Transcription Factor / Protein Engineering / Specificity / Synthetic Biology

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