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TitleCrystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms.
Journal, issue, pagesStructure, Vol. 30, Page 1088-1097.e3, Year 2022
Publish dateDec 14, 2021 (structure data deposition date)
AuthorsKohga, H. / Mori, T. / Tanaka, Y. / Yoshikaie, K. / Taniguchi, K. / Fujimoto, K. / Fritz, L. / Schneider, T. / Tsukazaki, T.
External linksStructure / PubMed:35660157
MethodsX-ray diffraction
Resolution2.35 - 2.8 Å
Structure data

PDB-7wag:
Crystal structure of MurJ squeezed form
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-7waw:
MurJ inward closed form
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-7wax:
MurJ inward occluded form
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

Chemicals

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

Source
  • escherichia coli k-12 (bacteria)
  • arsenophonus endosymbiont of nilaparvata lugens (Arsenophonus endosymbiont of Nilaparvata lugens)
KeywordsLIPID TRANSPORT / 14 transmembrane helices / inner membrane / Lipid II / MOP superfamily

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