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TitleStructures of nonsense-mediated mRNA decay factors UPF3B and UPF3A in complex with UPF2 reveal molecular basis for competitive binding and for neurodevelopmental disorder-causing mutation.
Journal, issue, pagesNucleic Acids Res., Vol. 50, Page 5934-5947, Year 2022
Publish dateMar 17, 2021 (structure data deposition date)
AuthorsBufton, J.C. / Powers, K.T. / Szeto, J.A. / Toelzer, C. / Berger, I. / Schaffitzel, C.
External linksNucleic Acids Res. / PubMed:35640974
MethodsX-ray diffraction
Resolution2.6 - 2.95 Å
Structure data

PDB-7nwu:
Co-crystal structure of UPF3B-RRM-NOPS-L with UPF2-MIF4GIII
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-7qg6:
Co-crystal structure of UPF3A-RRM-NOPS-L with UPF2-MIF4GIII
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

Chemicals

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-CL:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRNA BINDING PROTEIN / nonsense mediated mRNA decay neurological development x-linked intellectual disability up-frameshift proteins

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