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TitleTuning Enzyme Thermostability via Computationally Guided Covalent Stapling and Structural Basis of Enhanced Stabilization.
Journal, issue, pagesBiochemistry, Vol. 61, Page 1041-1054, Year 2022
Publish dateNov 2, 2021 (structure data deposition date)
AuthorsIannuzzelli, J.A. / Bacik, J.P. / Moore, E.J. / Shen, Z. / Irving, E.M. / Vargas, D.A. / Khare, S.D. / Ando, N. / Fasan, R.
External linksBiochemistry / PubMed:35612958
MethodsX-ray diffraction
Resolution1.17 - 1.7 Å
Structure data

PDB-7spe:
Crystal structure of sperm whale myoglobin variant sMb5(O2beY)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7spf:
Crystal structure of sperm whale myoglobin variant sMb5(pCaaF)
Method: X-RAY DIFFRACTION / Resolution: 1.17 Å

PDB-7spg:
Crystal structure of sperm whale myoglobin variant sMb13(pCaaF) in space group P212121
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-7sph:
Crystal structure of sperm whale myoglobin variant sMb13(pCaaF) in space group P21
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-NO3:
NITRATE ION

ChemComp-OXY:
OXYGEN MOLECULE

ChemComp-CL:
Unknown entry

Source
  • physeter catodon (sperm whale)
  • physeter macrocephalus (sperm whale)
KeywordsOXYGEN STORAGE / HEME / METALLOPROTEIN / THIOETHER / MYOGLOBIN

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