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Structure paper

TitleAntigen binding by conformational selection in near-germline antibodies.
Journal, issue, pagesJ. Biol. Chem., Vol. 298, Page 101901-101901, Year 2022
Publish dateNov 29, 2021 (structure data deposition date)
AuthorsBlackler, R.J. / Muller-Loennies, S. / Pokorny-Lehrer, B. / Legg, M.S.G. / Brade, L. / Brade, H. / Kosma, P. / Evans, S.V.
External linksJ. Biol. Chem. / PubMed:35395245
MethodsX-ray diffraction
Resolution1.3 - 2.15 Å
Structure data

PDB-7t0f:
Crystal structure of S25-39 Fab in complex with 4-MeO-KdoOAll
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7t0g:
Crystal structure of S25-39 Fab Unliganded 1
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-7t0h:
Crystal structure of S25-39 Fab Unliganded 2
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-7t0i:
Crystal structure of S25-39 Fab Unliganded 3
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7t0j:
Crystal structure of S25-2 Fab Unliganded 3
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7t0k:
Crystal structure of S25-2 Fab Unliganded 4
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-K71:
3-deoxy-4-O-methyl-alpha-D-manno-oct-2-ulopyranosonic acid

ChemComp-HOH:
WATER

ChemComp-BTB:
2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-BR:
BROMIDE ION

ChemComp-IOD:
IODIDE ION

Source
  • mus musculus (house mouse)
KeywordsIMMUNE SYSTEM / Antibody / Fab / Carbohydrate / Induced fit / Conformational selection

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