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Structure paper

TitleMERS-CoV ORF4b employs an unusual binding mechanism to target IMP alpha and block innate immunity.
Journal, issue, pagesNat Commun, Vol. 13, Page 1604-1604, Year 2022
Publish dateJul 14, 2021 (structure data deposition date)
AuthorsMunasinghe, T.S. / Edwards, M.R. / Tsimbalyuk, S. / Vogel, O.A. / Smith, K.M. / Stewart, M. / Foster, J.K. / Bosence, L.A. / Aragao, D. / Roby, J.A. ...Munasinghe, T.S. / Edwards, M.R. / Tsimbalyuk, S. / Vogel, O.A. / Smith, K.M. / Stewart, M. / Foster, J.K. / Bosence, L.A. / Aragao, D. / Roby, J.A. / Basler, C.F. / Forwood, J.K.
External linksNat Commun / PubMed:35338144
MethodsX-ray diffraction
Resolution1.95 - 2.6 Å
Structure data

PDB-7rfx:
Importin alpha2 in complex with MERS ORF4B
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7rfy:
Importin alpha3 in complex with MERS ORF4B
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7rfz:
Importin alpha 2 in complex with MERS ORF4B NLS peptide
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-7rg0:
Importin alpha2 in complex with MERS ORF4B R24A mutant
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7rg2:
Importin alpha2 in complex with MERS ORF4B R33A mutant
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7rg3:
Importin alpha2 in complex with MERS ORF4B R37A mutant
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7rg4:
Importin alpha2 in complex with p50 NLS
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-7rg5:
Importin alpha3 in complex with p50 NLS
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7rg6:
Importin alpha2 in complex with ORF4B Bat coronavirus HKU5
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • mus musculus (house mouse)
  • middle east respiratory syndrome-related coronavirus
  • homo sapiens (human)
  • bat coronavirus hku5
KeywordsTRANSPORT PROTEIN/Viral Protein / Complex / Transportin / Importin / Virus / TRANSPORT PROTEIN / TRANSPORT PROTEIN-Viral Protein complex / imortin / nuclear import

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