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TitleIn Vivo Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 144, Page 5614-5628, Year 2022
Publish dateFeb 21, 2022 (structure data deposition date)
AuthorsGao, L. / Meiring, J.C.M. / Varady, A. / Ruider, I.E. / Heise, C. / Wranik, M. / Velasco, C.D. / Taylor, J.A. / Terni, B. / Weinert, T. ...Gao, L. / Meiring, J.C.M. / Varady, A. / Ruider, I.E. / Heise, C. / Wranik, M. / Velasco, C.D. / Taylor, J.A. / Terni, B. / Weinert, T. / Standfuss, J. / Cabernard, C.C. / Llobet, A. / Steinmetz, M.O. / Bausch, A.R. / Distel, M. / Thorn-Seshold, J. / Akhmanova, A. / Thorn-Seshold, O.
External linksJ. Am. Chem. Soc. / PubMed:35290733
MethodsX-ray diffraction
Resolution1.82 - 2.36 Å
Structure data

PDB-7z01:
Z-SBTubA4 photoswitch bound to tubulin-DARPin D1 complex
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-7z02:
Z-SBTub2M photoswitch bound to tubulin-DARPin D1 complex
Method: X-RAY DIFFRACTION / Resolution: 2.36 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-I8R:
2-methoxy-5-[2-(5,6,7-trimethoxy-1,3-benzothiazol-2-yl)ethyl]phenol

ChemComp-HOH:
WATER

ChemComp-I8N:
6-methyl-2-[2-(3,4,5-trimethoxyphenyl)ethyl]-1,3-benzothiazole

Source
  • synthetic construct (others)
  • bos taurus (domestic cattle)
KeywordsCELL CYCLE / microtubule dynamics; cytoskeleton; photopharmacology; tubulin polymerisation inhibitor; optical control; colchicine; antimitotic; cell division; cell migration; development; photoswitch

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