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Structure paper

TitleStructural basis of dynamic P5CS filaments.
Journal, issue, pagesElife, Vol. 11, Year 2022
Publish dateMar 14, 2022
AuthorsJiale Zhong / Chen-Jun Guo / Xian Zhou / Chia-Chun Chang / Boqi Yin / Tianyi Zhang / Huan-Huan Hu / Guang-Ming Lu / Ji-Long Liu /
PubMed AbstractThe bifunctional enzyme Δ-pyrroline-5-carboxylate synthase (P5CS) is vital to the synthesis of proline and ornithine, playing an essential role in human health and agriculture. Pathogenic mutations ...The bifunctional enzyme Δ-pyrroline-5-carboxylate synthase (P5CS) is vital to the synthesis of proline and ornithine, playing an essential role in human health and agriculture. Pathogenic mutations in the P5CS gene (ALDH18A1) lead to neurocutaneous syndrome and skin relaxation connective tissue disease in humans, and P5CS deficiency seriously damages the ability to resist adversity in plants. We have recently found that P5CS forms cytoophidia in vivo and filaments in vitro. However, it is difficult to appreciate the function of P5CS filamentation without precise structures. Using cryo-electron microscopy, here we solve the structures of full-length P5CS in three states at resolution from 3.1 to 4.3 Å. We observe distinct ligand-binding states and conformational changes for the GK and GPR domains, respectively. Divergent helical filaments are assembled by P5CS tetramers and stabilized by multiple interfaces. Point mutations disturbing those interfaces prevent P5CS filamentation and greatly reduce the enzymatic activity. Our findings reveal that filamentation is crucial for the coordination between the GK and GPR domains, providing a structural basis for the catalytic function of P5CS filaments.
External linksElife / PubMed:35286254 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 4.3 Å
Structure data

31466

7f5t

EMDB-31466, PDB-7f5t:
Drosophila P5CS filament with glutamate
Method: EM (single particle) / Resolution: 4.1 Å

31467

7f5u

EMDB-31467, PDB-7f5u:
Drosophila P5CS filament with glutamate and ATPgammaS
Method: EM (single particle) / Resolution: 4.1 Å

31468

7f5v

EMDB-31468, PDB-7f5v:
Drosophila P5CS filament with glutamate, ATP, and NADPH
Method: EM (single particle) / Resolution: 3.6 Å

31469

7f5x

EMDB-31469, PDB-7f5x:
GK domain of Drosophila P5CS filament with glutamate
Method: EM (single particle) / Resolution: 3.5 Å

32875

7wx3

EMDB-32875, PDB-7wx3:
GK domain of Drosophila P5CS filament with glutamate, ATP, and NADPH
Method: EM (single particle) / Resolution: 3.1 Å

32876

7wx4

EMDB-32876, PDB-7wx4:
GK domain of Drosophila P5CS filament with glutamate and ATPgammaS
Method: EM (single particle) / Resolution: 3.4 Å

32877

7wxf

EMDB-32877, PDB-7wxf:
GPR domain of Drosophila P5CS filament with glutamate
Method: EM (single particle) / Resolution: 3.6 Å

32878

7wxg

EMDB-32878, PDB-7wxg:
GPR domain closed form of Drosophila P5CS filament with glutamate, ATP, and NADPH
Method: EM (single particle) / Resolution: 4.2 Å

32879

7wxh

EMDB-32879, PDB-7wxh:
GPR domain open form of Drosophila P5CS filament with glutamate, ATP, and NADPH
Method: EM (single particle) / Resolution: 4.3 Å

32880

7wxi

EMDB-32880, PDB-7wxi:
GPR domain of Drosophila P5CS filament with glutamate and ATPgammaS
Method: EM (single particle) / Resolution: 4.2 Å

Chemicals

ChemComp-GLU:
GLUTAMIC ACID / Glutamic acid

ChemComp-GGL:
GAMMA-L-GLUTAMIC ACID / Glutamic acid

ChemComp-RGP:
GAMMA-GLUTAMYL PHOSPHATE

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-MG:
Unknown entry

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

Source
  • drosophila melanogaster (fruit fly)
KeywordsTRANSFERASE / glutamate-bound / filament / ALDH18A1 / Delta-1-pyrroline-5-carboxylate synthase / BIOSYNTHETIC PROTEIN

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