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Structure paper

TitleConformational transitions and ligand-binding to a muscle-type nicotinic acetylcholine receptor.
Journal, issue, pagesNeuron, Vol. 110, Issue 8, Page 1358-11370.e5, Year 2022
Publish dateApr 20, 2022
AuthorsEleftherios Zarkadas / Eva Pebay-Peyroula / Mackenzie John Thompson / Guy Schoehn / Tomasz Uchański / Jan Steyaert / Christophe Chipot / Francois Dehez / John Edward Baenziger / Hugues Nury /
PubMed AbstractFast synaptic communication requires receptors that respond to the presence of neurotransmitter by opening an ion channel across the post-synaptic membrane. The muscle-type nicotinic acetylcholine ...Fast synaptic communication requires receptors that respond to the presence of neurotransmitter by opening an ion channel across the post-synaptic membrane. The muscle-type nicotinic acetylcholine receptor from the electric fish, Torpedo, is the prototypic ligand-gated ion channel, yet the structural changes underlying channel activation remain undefined. Here we use cryo-EM to solve apo and agonist-bound structures of the Torpedo nicotinic receptor embedded in a lipid nanodisc. Using both a direct biochemical assay to define the conformational landscape and molecular dynamics simulations to assay flux through the pore, we correlate structures with functional states and elucidate the motions that lead to pore activation of a heteromeric nicotinic receptor. We highlight an underappreciated role for the complementary subunit in channel gating, establish the structural basis for the differential agonist affinities of α/δ versus α /γ sites, and explain why nicotine is less potent at muscle nicotinic receptors compared to neuronal ones.
External linksNeuron / PubMed:35139364
MethodsEM (single particle)
Resolution2.5 - 3.23 Å
Structure data

14048

7qko

EMDB-14048, PDB-7qko:
Torpedo muscle-type nicotinic acetylcholine receptor - Resting conformation
Method: EM (single particle) / Resolution: 2.9 Å

14064

7ql5

EMDB-14064, PDB-7ql5:
Torpedo muscle-type nicotinic acetylcholine receptor - nicotine-bound conformation
Method: EM (single particle) / Resolution: 2.5 Å

14065

7ql6

EMDB-14065, PDB-7ql6:
Torpedo muscle-type nicotinic acetylcholine receptor - carbamylcholine-bound conformation
Method: EM (single particle) / Resolution: 3.23 Å

Chemicals

ChemComp-POV:
(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / phospholipid*YM

ChemComp-NCT:
(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE / alkaloid*YM

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

ChemComp-CCE:
2-[(AMINOCARBONYL)OXY]-N,N,N-TRIMETHYLETHANAMINIUM

Source
  • tetronarce californica (Pacific electric ray)
KeywordsMEMBRANE PROTEIN / pentameric ligand-gated ion channel / nicotinic receptor

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