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Structure paper

TitleA Morphing [4Fe-3S-nO]-Cluster within a Carbon Monoxide Dehydrogenase Scaffold.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 61, Page e202117000-e202117000, Year 2022
Publish dateDec 11, 2020 (structure data deposition date)
AuthorsJeoung, J.H. / Fesseler, J. / Domnik, L. / Klemke, F. / Sinnreich, M. / Teutloff, C. / Dobbek, H.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:35133707
MethodsX-ray diffraction
Resolution1.35 - 2.5 Å
Structure data

PDB-7b7q:
CooS-V with oxidized hybrid cluster
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-7b7t:
CooS-V with oxidized hybrid cluster
Method: X-RAY DIFFRACTION / Resolution: 1.38 Å

PDB-7b95:
CooS-V with partially oxidized hybrid cluster by hydroxylamine
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-7b97:
CooS-V with oxidized hybrid cluster by hydroxylamine for 30 min
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-7b9a:
CooS-V with Xe-soaked
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-T2N:
3,5-dioxa-7-thia-1-thionia-2$l^{2},4$l^{2},6$l^{3},8$l^{2}-tetraferrabicyclo[4.2.0]octane

ChemComp-TAM:
TRIS(HYDROXYETHYL)AMINOMETHANE / pH buffer*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-SF3:
FE4-S3 CLUSTER

ChemComp-BU3:
(R,R)-2,3-BUTANEDIOL

ChemComp-H2S:
HYDROSULFURIC ACID

ChemComp-XE:
XENON

ChemComp-BR:
BROMIDE ION

Source
  • carboxydothermus hydrogenoformans (strain atcc baa-161 / dsm 6008 / z-2901) (bacteria)
KeywordsOXIDOREDUCTASE / Hybrid cluster / HCP / CODH

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