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TitlePoint Mutations at a Key Site Alter the Cytochrome P450 OleP Structural Dynamics.
Journal, issue, pagesBiomolecules, Vol. 12, Year 2021
Publish dateNov 9, 2021 (structure data deposition date)
AuthorsMontemiglio, L.C. / Gugole, E. / Freda, I. / Exertier, C. / D'Auria, L. / Chen, C.G. / Nardi, A.N. / Cerutti, G. / Parisi, G. / D'Abramo, M. ...Montemiglio, L.C. / Gugole, E. / Freda, I. / Exertier, C. / D'Auria, L. / Chen, C.G. / Nardi, A.N. / Cerutti, G. / Parisi, G. / D'Abramo, M. / Savino, C. / Vallone, B.
External linksBiomolecules / PubMed:35053203
MethodsX-ray diffraction
Resolution2.08 - 2.7 Å
Structure data

PDB-7q6r:
OleP mutant E89Y in complex with 6DEB
Method: X-RAY DIFFRACTION / Resolution: 2.44 Å

PDB-7q6x:
OleP mutant S240Y in complex with 6DEB
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-7q89:
OleP mutant G92W in complex with 6DEB
Method: X-RAY DIFFRACTION / Resolution: 2.08 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-DEB:
6-DEOXYERYTHRONOLIDE B

ChemComp-GOL:
GLYCEROL

ChemComp-FMT:
FORMIC ACID

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

Source
  • streptomyces antibioticus (bacteria)
KeywordsOXIDOREDUCTASE / Cytochrome P450 / Oleandomicine / OleP / mutant / 6DEB

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