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TitleGene Fusion and Directed Evolution to Break Structural Symmetry and Boost Catalysis by an Oligomeric C-C Bond-Forming Enzyme.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 61, Page e202113970-e202113970, Year 2022
Publish dateSep 30, 2021 (structure data deposition date)
AuthorsXu, G. / Kunzendorf, A. / Crotti, M. / Rozeboom, H.J. / Thunnissen, A.W.H. / Poelarends, G.J.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:34890491
MethodsX-ray diffraction
Resolution2.35 Å
Structure data

PDB-7puo:
Structure of a fused 4-OT variant engineered for asymmetric Michael addition reactions
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • pseudomonas putida (bacteria)
KeywordsLYASE / Protein engineering / 4-oxalocrotonate tautomerase / Michael addition / carbon-carbon lyase

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