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Structure paper

TitleArchitecture of the chloroplast PSI-NDH supercomplex in Hordeum vulgare.
Journal, issue, pagesNature, Vol. 601, Issue 7894, Page 649-654, Year 2022
Publish dateDec 8, 2021
AuthorsLiangliang Shen / Kailu Tang / Wenda Wang / Chen Wang / Hangjun Wu / Zhiyuan Mao / Shaoya An / Shenghai Chang / Tingyun Kuang / Jian-Ren Shen / Guangye Han / Xing Zhang /
PubMed AbstractThe chloroplast NADH dehydrogenase-like (NDH) complex is composed of at least 29 subunits and has an important role in mediating photosystem I (PSI) cyclic electron transport (CET). The NDH complex ...The chloroplast NADH dehydrogenase-like (NDH) complex is composed of at least 29 subunits and has an important role in mediating photosystem I (PSI) cyclic electron transport (CET). The NDH complex associates with PSI to form the PSI-NDH supercomplex and fulfil its function. Here, we report cryo-electron microscopy structures of a PSI-NDH supercomplex from barley (Hordeum vulgare). The structures reveal that PSI-NDH is composed of two copies of the PSI-light-harvesting complex I (LHCI) subcomplex and one NDH complex. Two monomeric LHCI proteins, Lhca5 and Lhca6, mediate the binding of two PSI complexes to NDH. Ten plant chloroplast-specific NDH subunits are presented and their exact positions as well as their interactions with other subunits in NDH are elucidated. In all, this study provides a structural basis for further investigations on the functions and regulation of PSI-NDH-dependent CET.
External linksNature / PubMed:34879391
MethodsEM (single particle)
Resolution3.4 - 4.5 Å
Structure data

31307

7eu3

EMDB-31307, PDB-7eu3:
Chloroplast NDH complex
Method: EM (single particle) / Resolution: 3.7 Å

31348

7ew6

EMDB-31348, PDB-7ew6:
Barley photosystem I-LHCI-Lhca5 supercomplex
Method: EM (single particle) / Resolution: 3.4 Å

31350

7ewk

EMDB-31350, PDB-7ewk:
Barley photosystem I-LHCI-Lhca6 supercomplex
Method: EM (single particle) / Resolution: 3.88 Å

31498

7f9o

EMDB-31498, PDB-7f9o:
PSI-NDH supercomplex of Barley
Method: EM (single particle) / Resolution: 4.5 Å

Chemicals

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

Source
  • hordeum vulgare subsp. spontaneum (wild barley)
  • hordeum vulgare (barley)
KeywordsPHOTOSYNTHESIS / chloroplast NAD(P)H-quinone oxidoreductase / cyclic electron transport / Photosystem I / Light-harvesting complex / Lhca5 / Lhca6 / NDH

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