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Structure paper

TitleA newly introduced salt bridge cluster improves structural and biophysical properties of de novo TIM barrels.
Journal, issue, pagesProtein Sci., Vol. 31, Page 513-527, Year 2022
Publish dateJun 9, 2021 (structure data deposition date)
AuthorsKordes, S. / Romero-Romero, S. / Lutz, L. / Hocker, B.
External linksProtein Sci. / PubMed:34865275
MethodsX-ray diffraction
Resolution1.37 - 2.22 Å
Structure data

PDB-7osu:
sTIM11noCys-SB, a de novo designed TIM barrel with a salt-bridge cluster (crystal form 1)
Method: X-RAY DIFFRACTION / Resolution: 1.37 Å

PDB-7osv:
DeNovoTIM6-SB, a de novo designed TIM barrel with a salt-bridge cluster (crystal form 1)
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

PDB-7ot7:
sTIM11noCys-SB, a de novo designed TIM barrel with a salt-bridge cluster (crystal form 2)
Method: X-RAY DIFFRACTION / Resolution: 1.51 Å

PDB-7ot8:
DeNovoTIM6-SB, a de novo designed TIM barrel with a salt-bridge cluster (crystal form 2)
Method: X-RAY DIFFRACTION / Resolution: 2.22 Å

PDB-7p12:
DeNovoTIM13-SB, a de novo designed TIM barrel with a salt-bridge cluster
Method: X-RAY DIFFRACTION / Resolution: 1.69 Å

Chemicals

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-ACT:
ACETATE ION

ChemComp-PO4:
PHOSPHATE ION

Source
  • synthetic construct (others)
KeywordsDE NOVO PROTEIN / TIM barrel / salt bridge cluster

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