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-Structure paper
タイトル | Structural basis and mechanism of activation of two different families of G proteins by the same GPCR. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 28, Issue 11, Page 936-944, Year 2021 |
掲載日 | 2021年11月10日 |
著者 | Kamela O Alegre / Navid Paknejad / Minfei Su / Jian-Shu Lou / Jianyun Huang / Kelsey D Jordan / Edward T Eng / Joel R Meyerson / Richard K Hite / Xin-Yun Huang / |
PubMed 要旨 | The β-adrenergic receptor (β-AR) can activate two families of G proteins. When coupled to Gs, β-AR increases cardiac output, and coupling to Gi leads to decreased responsiveness in myocardial ...The β-adrenergic receptor (β-AR) can activate two families of G proteins. When coupled to Gs, β-AR increases cardiac output, and coupling to Gi leads to decreased responsiveness in myocardial infarction. By comparative structural analysis of turkey β-AR complexed with either Gi or Gs, we investigate how a single G-protein-coupled receptor simultaneously signals through two G proteins. We find that, although the critical receptor-interacting C-terminal α5-helices on Gα and Gα interact similarly with β-AR, the overall interacting modes between β-AR and G proteins vary substantially. Functional studies reveal the importance of the differing interactions and provide evidence that the activation efficacy of G proteins by β-AR is determined by the entire three-dimensional interaction surface, including intracellular loops 2 and 4 (ICL2 and ICL4). |
リンク | Nat Struct Mol Biol / PubMed:34759376 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.96 - 3.86 Å |
構造データ | EMDB-24789, PDB-7s0f: EMDB-24790, PDB-7s0g: |
化合物 | ChemComp-5FW: |
由来 |
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キーワード | SIGNALING PROTEIN / Gi protein / GPCR-Gi complex / agonist / chimera Gi/s protein / GPCR-Gi-s complex |