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Structure paper

TitleCoiled coils 9-to-5: rational de novo design of alpha-helical barrels with tunable oligomeric states.
Journal, issue, pagesChem Sci, Vol. 12, Page 6923-6928, Year 2021
Publish dateAug 14, 2020 (structure data deposition date)
AuthorsDawson, W.M. / Martin, F.J.O. / Rhys, G.G. / Shelley, K.L. / Brady, R.L. / Woolfson, D.N.
External linksChem Sci / PubMed:34745518
MethodsX-ray diffraction
Resolution1.1 - 1.77 Å
Structure data

PDB-7a1t:
A collapsed hexameric state of a de novo coiled-coil assembly: CC-Type2-(GgLaId)4-W19BrPhe.
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

PDB-7bas:
A de novo pentameric coiled-coil assembly: CC-Type2-(TgLaId)4-W19BrPhe.
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-7bat:
A hexameric barrel state of a de novo coiled-coil assembly: CC-Type2-(GgIaId)4
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-7bau:
A de novo pentameric coiled-coil assembly: CC-Type2-(TgIaId)4-W19BrPhe.
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

PDB-7bav:
A de novo pentameric coiled-coil assembly: CC-Type2-(TgLaId)4-W19BrPhe.
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-7baw:
A heptameric barrel state of a de novo coiled-coil assembly: CC-Type2-(GgIaId)4
Method: X-RAY DIFFRACTION / Resolution: 1.71 Å

PDB-7bim:
A de novo designed nonameric coiled coil, CC-Type2-(GgLaId)4
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

Chemicals

ChemComp-ACT:
ACETATE ION

ChemComp-CD:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-POL:
N-PROPANOL

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

Source
  • synthetic construct (others)
KeywordsDE NOVO PROTEIN / Coiled coil / synthetic peptide / homomeric assembly / alpha / helical / barrel / pentamer / hexamer / alpha-helical / alpha-helical barrel / nonamer / synthetic

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