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Structure paper

TitleA synthetic antibiotic class overcoming bacterial multidrug resistance.
Journal, issue, pagesNature, Vol. 599, Page 507-512, Year 2021
Publish dateAug 6, 2021 (structure data deposition date)
AuthorsMitcheltree, M.J. / Pisipati, A. / Syroegin, E.A. / Silvestre, K.J. / Klepacki, D. / Mason, J.D. / Terwilliger, D.W. / Testolin, G. / Pote, A.R. / Wu, K.J.Y. ...Mitcheltree, M.J. / Pisipati, A. / Syroegin, E.A. / Silvestre, K.J. / Klepacki, D. / Mason, J.D. / Terwilliger, D.W. / Testolin, G. / Pote, A.R. / Wu, K.J.Y. / Ladley, R.P. / Chatman, K. / Mankin, A.S. / Polikanov, Y.S. / Myers, A.G.
External linksNature / PubMed:34707295
MethodsX-ray diffraction
Resolution2.5 - 2.6 Å
Structure data

PDB-7rq8:
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.50A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7rq9:
Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.60A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-6IF:
methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-K:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
  • thermus thermophilus hb8 (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • escherichia virus t4
KeywordsRIBOSOME/RNA / Iboxamycin / clindamycin / lincosamides / structure-based drug design / antibiotic / resistance / methylation / A2058 / 23S rRNA / 70S ribosome / inhibition of translation / peptidyl transferase center / nascent peptide exit tunnel / RIBOSOME / RIBOSOME-RNA complex

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