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TitleThe novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity.
Journal, issue, pagesPlos Biol., Vol. 19, Page e3001428-e3001428, Year 2021
Publish dateAug 17, 2020 (structure data deposition date)
AuthorsForsberg, K.J. / Schmidtke, D.T. / Werther, R. / Uribe, R.V. / Hausman, D. / Sommer, M.O.A. / Stoddard, B.L. / Kaiser, B.K. / Malik, H.S.
External linksPlos Biol. / PubMed:34644300
MethodsX-ray diffraction
Resolution2.801 Å
Structure data

PDB-7jta:
Crystal structure of a putative nuclease with anti-Cas9 activity from an uncultured Clostridia bacterium
Method: X-RAY DIFFRACTION / Resolution: 2.801 Å

Chemicals

ChemComp-NO3:
NITRATE ION

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-HOH:
WATER

Source
  • clostridia bacterium (bacteria)
KeywordsUNKNOWN FUNCTION / Anti-CRISPR / nuclease / phage protein / Cas9

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