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TitleThe stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes.
Journal, issue, pagesScience, Vol. 374, Issue 6563, Page 52-57, Year 2021
Publish dateSep 30, 2021
AuthorsNgoc-Han Tran / Stephen D Carter / Ann De Mazière / Avi Ashkenazi / Judith Klumperman / Peter Walter / Grant J Jensen /
PubMed AbstractThe signaling network of the unfolded protein response (UPR) adjusts the protein-folding capacity of the endoplasmic reticulum (ER) according to need. The most conserved UPR sensor, IRE1α, spans the ...The signaling network of the unfolded protein response (UPR) adjusts the protein-folding capacity of the endoplasmic reticulum (ER) according to need. The most conserved UPR sensor, IRE1α, spans the ER membrane and activates through oligomerization. IRE1α oligomers accumulate in dynamic foci. We determined the in situ structure of IRE1α foci by cryogenic correlated light and electron microscopy combined with electron cryo-tomography and complementary immuno–electron microscopy in mammalian cell lines. IRE1α foci localized to a network of narrow anastomosing ER tubes (diameter, ~28 nm) with complex branching. The lumen of the tubes contained protein filaments, which were likely composed of arrays of IRE1α lumenal domain dimers that were arranged in two intertwined, left-handed helices. This specialized ER subdomain may play a role in modulating IRE1α signaling.
External linksScience / PubMed:34591618 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution15.0 - 30.0 Å
Structure data

EMDB-23058:
The stress-sensing domain of activated IRE1a forms helical filaments in narrow ER membrane tubes
Method: EM (subtomogram averaging) / Resolution: 15.0 Å

EMDB-23060:
The stress-sensing domain of activated IRE1a forms helical filaments in narrow ER membrane tubes
Method: EM (subtomogram averaging) / Resolution: 30.0 Å

Source
  • Homo sapiens (human)

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