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Structure paper

TitleMillisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection.
Journal, issue, pagesIucrj, Vol. 8, Page 784-792, Year 2021
Publish dateDec 17, 2020 (structure data deposition date)
AuthorsClinger, J.A. / Moreau, D.W. / McLeod, M.J. / Holyoak, T. / Thorne, R.E.
External linksIucrj / PubMed:34584739
MethodsX-ray diffraction
Resolution1.84 - 2.07 Å
Structure data

PDB-7l36:
PEPCK steady-state structure with Mn and GTP
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

PDB-7l3m:
PEPCK MMQX structure 40ms post-mixing with oxaloacetic acid
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-7l3v:
PEPCK MMQX structure 120ms post-mixing with oxaloacetic acid
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-PEP:
PHOSPHOENOLPYRUVATE

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-CO2:
CARBON DIOXIDE

Source
  • rattus norvegicus (Norway rat)
KeywordsLYASE / PEPCK / phosphoenolpyruvate carboxykinase / gluconeogensis / gluconeogenesis

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