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TitleCryo-EM structure of the sodium-driven chloride/bicarbonate exchanger NDCBE.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 5690, Year 2021
Publish dateSep 28, 2021
AuthorsWeiguang Wang / Kirill Tsirulnikov / Hristina R Zhekova / Gülru Kayık / Hanif Muhammad Khan / Rustam Azimov / Natalia Abuladze / Liyo Kao / Debbie Newman / Sergei Yu Noskov / Z Hong Zhou / Alexander Pushkin / Ira Kurtz /
PubMed AbstractSLC4 transporters play significant roles in pH regulation and cellular sodium transport. The previously solved structures of the outward facing (OF) conformation for AE1 (SLC4A1) and NBCe1 (SLC4A4) ...SLC4 transporters play significant roles in pH regulation and cellular sodium transport. The previously solved structures of the outward facing (OF) conformation for AE1 (SLC4A1) and NBCe1 (SLC4A4) transporters revealed an identical overall fold despite their different transport modes (chloride/bicarbonate exchange versus sodium-carbonate cotransport). However, the exact mechanism determining the different transport modes in the SLC4 family remains unknown. In this work, we report the cryo-EM 3.4 Å structure of the OF conformation of NDCBE (SLC4A8), which shares transport properties with both AE1 and NBCe1 by mediating the electroneutral exchange of sodium-carbonate with chloride. This structure features a fully resolved extracellular loop 3 and well-defined densities corresponding to sodium and carbonate ions in the tentative substrate binding pocket. Further, we combine computational modeling with functional studies to unravel the molecular determinants involved in NDCBE and SLC4 transport.
External linksNat Commun / PubMed:34584093 / PubMed Central
MethodsEM (single particle)
Resolution3.4 Å
Structure data

24683

7rtm

EMDB-24683, PDB-7rtm:
Cryo-EM Structure of the Sodium-driven Chloride/Bicarbonate Exchanger NDCBE (SLC4A8)
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-NA:
Unknown entry

ChemComp-CO3:
CARBONATE ION / Carbonate

Source
  • oryctolagus cuniculus (rabbit)
KeywordsMEMBRANE PROTEIN / NDCBE / sodium-driven chloride/bicarbonate exchanger / cryo-EM

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