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TitleActivation mechanism of human soluble guanylate cyclase by stimulators and activators.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 5492, Year 2021
Publish dateSep 17, 2021
AuthorsRui Liu / Yunlu Kang / Lei Chen /
PubMed AbstractSoluble guanylate cyclase (sGC) is the receptor for nitric oxide (NO) in human. It is an important validated drug target for cardiovascular diseases. sGC can be pharmacologically activated by ...Soluble guanylate cyclase (sGC) is the receptor for nitric oxide (NO) in human. It is an important validated drug target for cardiovascular diseases. sGC can be pharmacologically activated by stimulators and activators. However, the detailed structural mechanisms, through which sGC is recognized and positively modulated by these drugs at high spacial resolution, are poorly understood. Here, we present cryo-electron microscopy structures of human sGC in complex with NO and sGC stimulators, YC-1 and riociguat, and also in complex with the activator cinaciguat. These structures uncover the molecular details of how stimulators interact with residues from both β H-NOX and CC domains, to stabilize sGC in the extended active conformation. In contrast, cinaciguat occupies the haem pocket in the β H-NOX domain and sGC shows both inactive and active conformations. These structures suggest a converged mechanism of sGC activation by pharmacological compounds.
External linksNat Commun / PubMed:34535643 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 4.1 Å
Structure data

30618

7d9r

EMDB-30618, PDB-7d9r:
Structure of huamn soluble guanylate cyclase in the riociguat and NO-bound state
Method: EM (single particle) / Resolution: 3.7 Å

30619

7d9s

EMDB-30619, PDB-7d9s:
Structure of huamn soluble guanylate cyclase in the YC1 and NO-bound state
Method: EM (single particle) / Resolution: 3.9 Å

30620

7d9t

EMDB-30620, PDB-7d9t:
Structure of human soluble guanylate cyclase in the cinciguat-bound inactive state
Method: EM (single particle) / Resolution: 4.1 Å

30621

7d9u

EMDB-30621, PDB-7d9u:
Structure of human soluble guanylate cyclase in the cinciguat-bound activated state
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-GZO:
methyl ~{N}-[4,6-bis(azanyl)-2-[1-[(2-fluorophenyl)methyl]pyrazolo[3,4-b]pyridin-3-yl]pyrimidin-5-yl]-~{N}-methyl-carbamate / medication*YM / Riociguat

ChemComp-YC1:
[5-[1-(phenylmethyl)indazol-3-yl]furan-2-yl]methanol

ChemComp-Z90:
4-({(4-carboxybutyl)[2-(2-{[4-(2-phenylethyl)benzyl]oxy}phenyl)ethyl]amino}methyl)benzoic acid / Cinaciguat

Source
  • homo sapiens (human)
KeywordsSIGNALING PROTEIN / soluble guanylate cyclase

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