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TitleCryoEM structure of the super-constricted two-start dynamin 1 filament.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 5393, Year 2021
Publish dateSep 13, 2021
AuthorsJiwei Liu / Frances Joan D Alvarez / Daniel K Clare / Jeffrey K Noel / Peijun Zhang /
PubMed AbstractDynamin belongs to the large GTPase superfamily, and mediates the fission of vesicles during endocytosis. Dynamin molecules are recruited to the neck of budding vesicles to assemble into a helical ...Dynamin belongs to the large GTPase superfamily, and mediates the fission of vesicles during endocytosis. Dynamin molecules are recruited to the neck of budding vesicles to assemble into a helical collar and to constrict the underlying membrane. Two helical forms were observed: the one-start helix in the constricted state and the two-start helix in the super-constricted state. Here we report the cryoEM structure of a super-constricted two-start dynamin 1 filament at 3.74 Å resolution. The two strands are joined by the conserved GTPase dimeric interface. In comparison with the one-start structure, a rotation around Hinge 1 is observed, essential for communicating the chemical power of the GTPase domain and the mechanical force of the Stalk and PH domain onto the underlying membrane. The Stalk interfaces are well conserved and serve as fulcrums for adapting to changing curvatures. Relative to one-start, small rotations per interface accumulate to bring a drastic change in the helical pitch. Elasticity theory rationalizes the diversity of dynamin helical symmetries and suggests corresponding functional significance.
External linksNat Commun / PubMed:34518553 / PubMed Central
MethodsEM (helical sym.)
Resolution3.74 Å
Structure data

11932

7ax3

EMDB-11932, PDB-7ax3:
CryoEM structure of the super-constricted two-start dynamin 1 filament
Method: EM (helical sym.) / Resolution: 3.74 Å

Chemicals

ChemComp-GCP:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-PCP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
KeywordsCYTOSOLIC PROTEIN / DYNAMIN

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