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-Structure paper
Title | A human apolipoprotein L with detergent-like activity kills intracellular pathogens. |
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Journal, issue, pages | Science, Vol. 373, Issue 6552, Year 2021 |
Publish date | Jul 16, 2021 |
Authors | Ryan G Gaudet / Shiwei Zhu / Anushka Halder / Bae-Hoon Kim / Clinton J Bradfield / Shuai Huang / Dijin Xu / Agnieszka Mamiñska / Thanh Ngoc Nguyen / Michael Lazarou / Erdem Karatekin / Kallol Gupta / John D MacMicking / |
PubMed Abstract | Activation of cell-autonomous defense by the immune cytokine interferon-γ (IFN-γ) is critical to the control of life-threatening infections in humans. IFN-γ induces the expression of hundreds of ...Activation of cell-autonomous defense by the immune cytokine interferon-γ (IFN-γ) is critical to the control of life-threatening infections in humans. IFN-γ induces the expression of hundreds of host proteins in all nucleated cells and tissues, yet many of these proteins remain uncharacterized. We screened 19,050 human genes by CRISPR-Cas9 mutagenesis and identified IFN-γ-induced apolipoprotein L3 (APOL3) as a potent bactericidal agent protecting multiple non-immune barrier cell types against infection. Canonical apolipoproteins typically solubilize mammalian lipids for extracellular transport; APOL3 instead targeted cytosol-invasive bacteria to dissolve their anionic membranes into human-bacterial lipoprotein nanodiscs detected by native mass spectrometry and visualized by single-particle cryo-electron microscopy. Thus, humans have harnessed the detergent-like properties of extracellular apolipoproteins to fashion an intracellular lysin, thereby endowing resident nonimmune cells with a mechanism to achieve sterilizing immunity. |
External links | Science / PubMed:34437126 / PubMed Central |
Methods | EM (single particle) |
Resolution | 15.0 Å |
Structure data | EMDB-24144: |
Source |
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