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TitleDiscovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection.
Journal, issue, pagesAngew Chem Int Ed Engl, Vol. 60, Issue 39, Page 21211-21215, Year 2021
Publish dateSep 20, 2021
AuthorsNataly Kacherovsky / Lucy F Yang / Ha V Dang / Emmeline L Cheng / Ian I Cardle / Alexandra C Walls / Matthew McCallum / Drew L Sellers / Frank DiMaio / Stephen J Salipante / Davide Corti / David Veesler / Suzie H Pun /
PubMed AbstractThe coronavirus disease 2019 (COVID-19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for ...The coronavirus disease 2019 (COVID-19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for distinct viral proteins are critical components for rapid diagnostics and targeted therapeutics. In this work, we demonstrate the selection of novel DNA aptamers that bind to the SARS-CoV-2 spike glycoprotein with high specificity and affinity (<80 nM). Through binding assays and high resolution cryo-EM, we demonstrate that SNAP1 (SARS-CoV-2 spike protein N-terminal domain-binding aptamer 1) binds to the S N-terminal domain. We applied SNAP1 in lateral flow assays (LFAs) and ELISAs to detect UV-inactivated SARS-CoV-2 at concentrations as low as 5×10  copies mL . SNAP1 is therefore a promising molecular tool for SARS-CoV-2 diagnostics.
External linksAngew Chem Int Ed Engl / PubMed:34328683 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.6 Å
Structure data

EMDB-24678: SARS-CoV-2 S/S2M11/SNAP1 Global Refinement
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-24679: SARS-CoV-2 S/S2M11/SNAP1 Local Refinement
Method: EM (single particle) / Resolution: 3.6 Å

Source
  • Homo sapiens (human)

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