EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for genome packaging, retention, and ejection in human cytomegalovirus.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 4538, Year 2021
掲載日	2021年7月27日
著者	Zhihai Li / Jingjing Pang / Lili Dong / Xuekui Yu /
PubMed 要旨	How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched ...How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV. The 5-fold symmetric 10-helix anchor-uncommon among known portals-contacts the portal-encircling DNA, which is presumed to squeeze the portal as the genome packaging proceeds. We surmise that the 10-helix anchor dampens this action to delay the portal reaching a "head-full" packaging state, thus facilitating the large genome to be packaged. The 6-fold symmetric turret, latched via a coiled coil to a helix from a major capsid protein, supports the portal to retain the packaged genome. CVSCs at the penton vertices-presumed to increase inner capsid pressure-display a low stoichiometry, which would aid genome retention. We also demonstrate that the portal and capsid undergo conformational changes to facilitate genome ejection after viral cell entry.
リンク	Nat Commun / PubMed:34315863 / PubMed Central
手法	EM (単粒子)
解像度	4.0 - 6.8 Å
構造データ	EMDB-31290: Human Cytomegalovirus, the reconstruction of C1 portal vertex in the enveloped capsid 手法: EM (単粒子) / 解像度: 5.5 Å EMDB-31291: Human Cytomegalovirus, the reconstruction of C1 portal vertex in the partially-enveloped capsid 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-31292: Human Cytomegalovirus, the reconstruction of C1 capsid in the enveloped capsid 手法: EM (単粒子) / 解像度: 6.8 Å EMDB-31293: Human Cytomegalovirus, the reconstruction of C1 capsid in the partially-enveloped capsid 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-31295: Human Cytomegalovirus, the reconstruction of C12 portal in the enveloped capsid 手法: EM (単粒子) / 解像度: 4.5 Å 31296 7et2 EMDB-31296, PDB-7et2: Human Cytomegalovirus, C12 portal 手法: EM (単粒子) / 解像度: 4.2 Å 31297 7et3 EMDB-31297, PDB-7et3: C5 portal vertex in the enveloped virion capsid 手法: EM (単粒子) / 解像度: 4.2 Å 31298 7etj EMDB-31298, PDB-7etj: C5 portal vertex in the partially-enveloped virion capsid 手法: EM (単粒子) / 解像度: 4.0 Å 31299 7etm EMDB-31299, PDB-7etm: C6 portal vertex in the enveloped virion capsid 手法: EM (単粒子) / 解像度: 5.9 Å EMDB-31300: Human Cytomegalovirus, C6 reconstruction of the portal vertex in partially-enveloped capsid 手法: EM (単粒子) / 解像度: 5.3 Å 31301 7eto EMDB-31301, PDB-7eto: C1 CVSC-binding penton vertex in the virion capsid of Human Cytomegalovirus 手法: EM (単粒子) / 解像度: 4.0 Å
由来	human cytomegalovirus (ヘルペスウイルス) HHV-5, Human herpesvirus 5
キーワード	VIRAL PROTEIN / C12 portal / partially-enveloped capsid / Human Cytomegalovirus / C5 portal vertex / enveloped capsid / C6 portal vertex / C1 CVSC-binding penton vertex / virion capsid