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Title | Structural basis for genome packaging, retention, and ejection in human cytomegalovirus. |
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Journal, issue, pages | Nat Commun, Vol. 12, Issue 1, Page 4538, Year 2021 |
Publish date | Jul 27, 2021 |
![]() | Zhihai Li / Jingjing Pang / Lili Dong / Xuekui Yu / ![]() |
PubMed Abstract | How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched ...How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV. The 5-fold symmetric 10-helix anchor-uncommon among known portals-contacts the portal-encircling DNA, which is presumed to squeeze the portal as the genome packaging proceeds. We surmise that the 10-helix anchor dampens this action to delay the portal reaching a "head-full" packaging state, thus facilitating the large genome to be packaged. The 6-fold symmetric turret, latched via a coiled coil to a helix from a major capsid protein, supports the portal to retain the packaged genome. CVSCs at the penton vertices-presumed to increase inner capsid pressure-display a low stoichiometry, which would aid genome retention. We also demonstrate that the portal and capsid undergo conformational changes to facilitate genome ejection after viral cell entry. |
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Methods | EM (single particle) |
Resolution | 4.0 - 6.8 Å |
Structure data | ![]() EMDB-31290: ![]() EMDB-31291: ![]() EMDB-31292: ![]() EMDB-31293: ![]() EMDB-31295: EMDB-31296, PDB-7et2: EMDB-31297, PDB-7et3: EMDB-31298, PDB-7etj: EMDB-31299, PDB-7etm: ![]() EMDB-31300: EMDB-31301, PDB-7eto: |
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