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TitleA plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental gene partitioning in Toxoplasma .
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateFeb 8, 2021 (structure data deposition date)
AuthorsFarhat, D.C. / Bowler, M.W. / Communie, G. / Pontier, D. / Belmudes, L. / Mas, C. / Corrao, C. / Coute, Y. / Bougdour, A. / Lagrange, T. ...Farhat, D.C. / Bowler, M.W. / Communie, G. / Pontier, D. / Belmudes, L. / Mas, C. / Corrao, C. / Coute, Y. / Bougdour, A. / Lagrange, T. / Hakimi, M.A. / Swale, C.
External linksElife / PubMed:34263725
MethodsX-ray diffraction
Resolution1.23 - 1.38 Å
Structure data

PDB-7ng2:
Crystal structure of Toxoplasma CPSF4-YTH domain in apo form
Method: X-RAY DIFFRACTION / Resolution: 1.23 Å

PDB-7nh2:
Crystal structure of Toxoplasma CPSF4-YTH domain bound to m6A
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-7njc:
Crystal structure of the Toxoplasma CPSF4 YTH-domain in complex with a 7 mer m6A-modified RNA
Method: X-RAY DIFFRACTION / Resolution: 1.38 Å

Chemicals

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-HOH:
WATER

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-OYK:
~{N},9-dimethylpurin-6-amine

ChemComp-TOE:
2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL

ChemComp-6MD:
N-methyladenosine

Source
  • toxoplasma gondii (strain atcc 50611 / me49) (eukaryote)
  • synthetic construct (others)
KeywordsRNA BINDING PROTEIN / YTH / Cleavage and Polyadenylation / RNA / m6A / RNA recognition protein

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