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Structure paper

TitlePromoter-sequence determinants and structural basis of primer-dependent transcription initiation in Escherichia coli .
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 118, Year 2021
Publish dateMar 27, 2021 (structure data deposition date)
AuthorsSkalenko, K.S. / Li, L. / Zhang, Y. / Vvedenskaya, I.O. / Winkelman, J.T. / Cope, A.L. / Taylor, D.M. / Shah, P. / Ebright, R.H. / Kinney, J.B. / Nickels, B.E.
External linksProc. Natl. Acad. Sci. USA / PubMed:34187896
MethodsX-ray diffraction
Resolution2.808 - 3.34 Å
Structure data

PDB-7eh0:
Thermus thermophilus RNA polymerase transcription initiation complex containing a template-strand purine at position TSS-2, UpA RNA primer and CMPcPP
Method: X-RAY DIFFRACTION / Resolution: 2.808 Å

PDB-7eh1:
Thermus thermophilus transcription initiation complex containing a template-strand purine at position TSS-2, GpG RNA primer, and CMPcPP
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-7eh2:
Thermus thermophilus transcription initiation complex containing a template-strand pyrimidine at position TSS-2 and GpG RNA primer
Method: X-RAY DIFFRACTION / Resolution: 3.34 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-2TM:
5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine

ChemComp-HOH:
WATER

ChemComp-BU1:
1,4-BUTANEDIOL

Source
  • thermus thermophilus hb8 (bacteria)
  • synthetic construct (others)
KeywordsTRANSCRIPTION/DNA/RNA / Thermus thermophilus / RNA polymerase / transcription initiation / UpA / CMPcPP / TRANSCRIPTION / TRANSCRIPTION-DNA-RNA complex / GpG

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