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Structure paper

TitleEngineering the protein dynamics of an ancestral luciferase.
Journal, issue, pagesNat Commun, Vol. 12, Page 3616-3616, Year 2021
Publish dateJul 3, 2019 (structure data deposition date)
AuthorsSchenkmayerova, A. / Pinto, G.P. / Toul, M. / Marek, M. / Hernychova, L. / Planas-Iglesias, J. / Daniel Liskova, V. / Pluskal, D. / Vasina, M. / Emond, S. ...Schenkmayerova, A. / Pinto, G.P. / Toul, M. / Marek, M. / Hernychova, L. / Planas-Iglesias, J. / Daniel Liskova, V. / Pluskal, D. / Vasina, M. / Emond, S. / Dorr, M. / Chaloupkova, R. / Bednar, D. / Prokop, Z. / Hollfelder, F. / Bornscheuer, U.T. / Damborsky, J.
External linksNat Commun / PubMed:34127663
MethodsX-ray diffraction
Resolution1.9 - 2.001 Å
Structure data

PDB-6s6e:
Crystal structure of the engineered ancestor of haloalkane dehalogenases and Renilla luciferase (AncHLD-RLuc I161_F162PinsL)
Method: X-RAY DIFFRACTION / Resolution: 2.001 Å

PDB-6s97:
Fragment transplantation onto a hyperstable ancestor of haloalkane dehalogenases and Renilla luciferase (Anc-FT)
Method: X-RAY DIFFRACTION / Resolution: 1.953 Å

PDB-6yn2:
Crystal structure of Renilla reniformis luciferase variant RLuc8-W121F/E144Q in complex with a coelenteramide (the postcatalytic enzyme-product complex)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-CEI:
N-[3-BENZYL-5-(4-HYDROXYPHENYL)PYRAZIN-2-YL]-2-(4-HYDROXYPHENYL)ACETAMIDE

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-K:
Unknown entry

Source
  • synthetic construct (others)
  • renilla reniformis (sea pansy)
KeywordsLUMINESCENT PROTEIN / engineered ancestral enzyme / bioluminscence / coelenterazine-utilizing enzyme / fragment transplantation / ancestral enzyme / coelenteramide-bound enzyme

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