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TitleTargeting immunodominant Bet v 1 epitopes with monoclonal antibodies prevents the birch allergic response.
Journal, issue, pagesJ Allergy Clin Immunol, Vol. 149, Issue 1, Page 200-211, Year 2022
Publish dateJun 11, 2021
AuthorsAmanda Atanasio / Matthew C Franklin / Vishal Kamat / Annabel Romero Hernandez / Ashok Badithe / Li-Hong Ben / Jennifer Jones / Joannie Bautista / George D Yancopoulos / William Olson / Andrew J Murphy / Matthew A Sleeman / Jamie M Orengo
PubMed AbstractBACKGROUND: Blocking the major cat allergen, Fel d 1, with mAbs was effective in preventing an acute cat allergic response.OBJECTIVES: This study sought to extend the allergen-specific antibody ...BACKGROUND: Blocking the major cat allergen, Fel d 1, with mAbs was effective in preventing an acute cat allergic response.
OBJECTIVES: This study sought to extend the allergen-specific antibody approach and demonstrate that a combination of mAbs targeting Bet v 1, the immunodominant and most abundant allergenic protein in birch pollen, can prevent the birch allergic response.
METHODS: Bet v 1-specific mAbs, REGN5713, REGN5714, and REGN5715, were isolated using the VelocImmune platform. Surface plasmon resonance, x-ray crystallography, and cryo-electron microscopy determined binding kinetics and structural data. Inhibition of IgE-binding, basophil activation, and mast cell degranulation were assessed via blocking ELISA, flow cytometry, and the passive cutaneous anaphylaxis mouse model.
RESULTS: REGN5713, REGN5714, and REGN5715 bind with high affinity and noncompetitively to Bet v 1. A cocktail of all 3 antibodies, REGN5713/14/15, blocks IgE binding to Bet v 1 and inhibits Bet v 1- and birch pollen extract-induced basophil activation ex vivo and mast cell degranulation in vivo. Crystal structures of the complex of Bet v 1 with immunoglobulin antigen-binding fragments of REGN5713 or REGN5715 show distinct interaction sites on Bet v 1. Cryo-electron microscopy reveals a planar and roughly symmetrical complex formed by REGN5713/14/15 bound to Bet v 1.
CONCLUSIONS: These data confirm the immunodominance of Bet v 1 in birch allergy and demonstrate blockade of the birch allergic response with REGN5713/14/15. Structural analyses show simultaneous binding of REGN5713, REGN5714, and REGN5715 with substantial areas of Bet v 1 exposed, suggesting that targeting specific epitopes is sufficient to block the allergic response.
External linksJ Allergy Clin Immunol / PubMed:34126155
MethodsEM (single particle) / X-ray diffraction
Resolution3 - 3.71 Å
Structure data

24073

7mxl

EMDB-24073, PDB-7mxl:
Complex of Bet v 1 with the Fab fragments of a three antibody cocktail
Method: EM (single particle) / Resolution: 3.2 Å

PDB-7n0u:
Complex of recombinant Bet v 1 with Fab fragment of REGN5713
Method: X-RAY DIFFRACTION / Resolution: 3.0 Å

PDB-7n0v:
Complex of recombinant Bet v 1 with Fab fragment of REGN5715
Method: X-RAY DIFFRACTION / Resolution: 3.71 Å

Chemicals

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-HOH:
WATER / Water

Source
  • betula pendula (European white birch)
  • homo sapiens (human)
  • European white birch, Betula verrucosa
  • mus musculus (house mouse)
KeywordsALLERGEN / COMPLEX / ANTIBODY / BIRCH POLLEN ALLERGEN BET V 1 / Birch pollen / allergy / neutralizing antibody / immunotherapy

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