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Structure paper

TitleDecoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst.
Journal, issue, pagesChem Sci, Vol. 11, Page 11162-11178, Year 2020
Publish dateAug 30, 2019 (structure data deposition date)
AuthorsMarkova, K. / Chmelova, K. / Marques, S.M. / Carpentier, P. / Bednar, D. / Damborsky, J. / Marek, M.
External linksChem Sci / PubMed:34094357
MethodsX-ray diffraction
Resolution1.55 - 1.6 Å
Structure data

PDB-6sp5:
Structure of hyperstable haloalkane dehalogenase variant DhaA115
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6sp8:
Structure of hyperstable haloalkane dehalogenase variant DhaA115 prepared by the 'soak-and-freeze' method under 150 bar of krypton pressure
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

Chemicals

ChemComp-B3P:
2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-SCN:
THIOCYANATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-KR:
KRYPTON

Source
  • rhodococcus rhodochrous (bacteria)
KeywordsHYDROLASE / Haloalkane dehalogenase

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