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-Structure paper
Title | Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions. |
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Journal, issue, pages | Biochem J, Vol. 478, Issue 12, Page 2371-2384, Year 2021 |
Publish date | Jun 25, 2021 |
Authors | Ido Caspy / Mariia Fadeeva / Sebastian Kuhlgert / Anna Borovikova-Sheinker / Daniel Klaiman / Gal Masrati / Friedel Drepper / Nir Ben-Tal / Michael Hippler / Nathan Nelson / |
PubMed Abstract | Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and ...Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI-Pc ternary complex, leading to exclusion of water molecules from PsaA-PsaB/Pc interface once the PSI-Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover. |
External links | Biochem J / PubMed:34085703 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.74 Å |
Structure data | EMDB-11326, PDB-6zoo: |
Chemicals | ChemComp-CL0: ChemComp-CLA: ChemComp-PQN: ChemComp-SF4: ChemComp-BCR: ChemComp-LHG: ChemComp-LMT: ChemComp-LMG: ChemComp-CA: ChemComp-DGD: ChemComp-LUT: ChemComp-CHL: ChemComp-XAT: ChemComp-3PH: ChemComp-CU: ChemComp-HOH: |
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Keywords | PHOTOSYNTHESIS / Photosystem I / membrane protein |